Chromatin Dynamics through the Lens of Nucleosomes

The crucial role of nucleosomes in chromatin function relies on their dynamics. We characterize the dynamics of nucleosomes and their complexes with other proteins including linker histones (chromatosomes). The questions of interest include. How do binding partners recognize DNA motifs and histones in the nucleosomal context. What are the effects of DNA methylation on nucleosome dynamics and stability. How do disordered histone tails may modulate the DNA accessibility and binding to nucleosomes of other macromolecules? To aid in characterization of experimentally unknown nucleosome related systems, we identify sequence and functional features and use experimental data to build high resolution structural models of canonical, histone variant nucleosomes and chromatosomes with the native DNA sequences.